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From SpongePedia, the First SpongeBob Wiki.
The Jellyfish Queen is the big version of a normal Jellyfish and has powerful stingers. A robot version appeared in episode I'm Your Biggest Fanatic that was made by Kevin the Sea Cucumber and his Anchovy friends.
In episode: Jellyfishing, the real version was shown and stung Squidward twice. She also appears on the WhoBob WhatPants? DVD on one of the 4 Shorts, and then stings Patrick.